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Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans
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JoVE Journal 発生生物学
Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans

Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans

5,848 Views

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06:18 min

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April 13, 2018

DOI:

10.3791/57414-v

DOI:
10.3791/57414-v

•

06:18 min
April 13, 2018

•

3 Views
,
1生物学科,University of Saskatchewan

概要

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A step-by-step guide to probe loss of lysosomal acidity in the intestine of C. elegans using the pH-sensitive vital dye 5(6)-carboxy-2',7'-dichlorofluorescein diacetate (cDCFDA)

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Cite this Article

Baxi, K., de Carvalho, C. E. Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans. J. Vis. Exp. (134), e57414, doi:10.3791/57414 (2018).

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