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Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans
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JoVE 杂志 发育生物学
Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans

Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans

DOI:
10.3791/57414-v

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06:18 min

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April 13, 2018

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,
1Department of Biology,University of Saskatchewan

Chapters

  • 00:04Title
  • 01:01Staining and Imaging of Intestinal Lysosomes
  • 04:24Results: Study of the Lysosomal Functional Activity Using a pH Sensitive Dye
  • 05:38Conclusion

Summary

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A step-by-step guide to probe loss of lysosomal acidity in the intestine of C. elegans using the pH-sensitive vital dye 5(6)-carboxy-2',7'-dichlorofluorescein diacetate (cDCFDA)

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Keywords: Lysosomal AlkalinizationCaenorhabditis ElegansAutophagyLysosomal FunctionProtein DegradationCDCFDA StainingOP50 BacteriaNGM PlatesM9 BufferAgarose PadSodium Azide

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