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Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans

Assessing Lysosomal Alkalinization in the Intestine of Live Caenorhabditis elegans

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06:18 min

April 13, 2018

DOI:

06:18 min
April 13, 2018

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Summary

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A step-by-step guide to probe loss of lysosomal acidity in the intestine of C. elegans using the pH-sensitive vital dye 5(6)-carboxy-2',7'-dichlorofluorescein diacetate (cDCFDA)

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