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Legame dell'anticorpo Specificità per Kappa (Vκ) umani (IgM) Anticorpi catena contenenti chiari: Polysialic Acid (PSA) Allegato alla NCAM come caso di studio
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Legame dell'anticorpo Specificità per Kappa (Vκ) umani (IgM) Anticorpi catena contenenti chiari: Polysialic Acid (PSA) Allegato alla NCAM come caso di studio
Bölümler
- 00:05Başlık
- 01:09General CNS Tissue Preparation
- 02:42Protein L Agarose Bead Preparation
- 03:43Antibody-Antigen-Agarose L Complex Formation and Antigen Detection in Western Blots
- 08:44Results: Antibody Binding Specificity for Kappa Light Chain-containing Human Antibodies
- 09:54Conclusion
We present a protocol to identify protein moieties containing antigens for human and mouse IgM antibodies with specific kappa (Vκ) light chains. This protocol is not limited to IgM class antibodies but applies to all immunoglobulin isotypes that target their antigen with sufficiently high affinity during immunoprecipitations.
