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カッパ（Vκ）に対する抗体の結合特異性軽鎖を含むヒト（IgM抗体）抗体：ポリシアル酸（PSA）ケーススタディとしてNCAMに添付
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study
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カッパ（Vκ）に対する抗体の結合特異性軽鎖を含むヒト（IgM抗体）抗体：ポリシアル酸（PSA）ケーススタディとしてNCAMに添付

13,836 Views

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11:10 min

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June 29, 2016

DOI:

10.3791/54139-v

DOI:
10.3791/54139-v

•

11:10 min
June 29, 2016

•

1 Views
, , , , , ,
1Department of Neurology,Mayo Klinik, 2Mayo Clinic Center for Multiple Sclerosis and Autoimmune Neurology,Mayo Klinik, 3Center for Regenerative Medicine, Neuroregeneration,Mayo Klinik, 4Division of Neonatal Medicine,Mayo Klinik, 5Department of Pediatric and Adolescent Medicine,Mayo Klinik

Özet

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We present a protocol to identify protein moieties containing antigens for human and mouse IgM antibodies with specific kappa (Vκ) light chains. This protocol is not limited to IgM class antibodies but applies to all immunoglobulin isotypes that target their antigen with sufficiently high affinity during immunoprecipitations.

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Cite this Article

Watzlawik, J. O., Kahoud, R. J., Wootla, B., Painter, M. M., Warrington, A. E., Carey, W. A., Rodriguez, M. Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study. J. Vis. Exp. (112), e54139, doi:10.3791/54139 (2016).

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