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Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study

Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study

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13,848 Views

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11:10 min

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June 29, 2016

DOI:

10.3791/54139-v

DOI:
10.3791/54139-v

•

11:10 min
June 29, 2016

•

1 Views
, , , , , ,
1Department of Neurology,Mayo Clinic, 2Mayo Clinic Center for Multiple Sclerosis and Autoimmune Neurology,Mayo Clinic, 3Center for Regenerative Medicine, Neuroregeneration,Mayo Clinic, 4Division of Neonatal Medicine,Mayo Clinic, 5Department of Pediatric and Adolescent Medicine,Mayo Clinic

Summary

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We present a protocol to identify protein moieties containing antigens for human and mouse IgM antibodies with specific kappa (Vκ) light chains. This protocol is not limited to IgM class antibodies but applies to all immunoglobulin isotypes that target their antigen with sufficiently high affinity during immunoprecipitations.

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Cite this Article

Watzlawik, J. O., Kahoud, R. J., Wootla, B., Painter, M. M., Warrington, A. E., Carey, W. A., Rodriguez, M. Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study. J. Vis. Exp. (112), e54139, doi:10.3791/54139 (2016).

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