Journal
/
免疫与感染
/
Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study
JoVE 杂志
免疫与感染
This content is Free Access.
JoVE 杂志 免疫与感染
Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study

Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study

13,848 Views

•

11:10 min

•

June 29, 2016

DOI:

10.3791/54139-v

DOI:
10.3791/54139-v

•

11:10 min
June 29, 2016

•

1 Views
, , , , , ,
1Department of Neurology,Mayo Clinic, 2Mayo Clinic Center for Multiple Sclerosis and Autoimmune Neurology,Mayo Clinic, 3Center for Regenerative Medicine, Neuroregeneration,Mayo Clinic, 4Division of Neonatal Medicine,Mayo Clinic, 5Department of Pediatric and Adolescent Medicine,Mayo Clinic

Summary

Automatically generated

We present a protocol to identify protein moieties containing antigens for human and mouse IgM antibodies with specific kappa (Vκ) light chains. This protocol is not limited to IgM class antibodies but applies to all immunoglobulin isotypes that target their antigen with sufficiently high affinity during immunoprecipitations.

Explore More Videos

Antibody BindingKappa Light ChainIgM AntibodiesPolysialic AcidNCAMAntigen IdentificationTherapeutic PotentialIgM IsotypeBiomarkersImmunotherapeutic Agents癌症研究Central Nervous SystemLysateImmunoprecipitation

Read Article

Cite this Article

Watzlawik, J. O., Kahoud, R. J., Wootla, B., Painter, M. M., Warrington, A. E., Carey, W. A., Rodriguez, M. Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study. J. Vis. Exp. (112), e54139, doi:10.3791/54139 (2016).

Download .ris file

Copy

Share Video

.

Copy