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Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study

Antibody Binding Specificity for Kappa (Vκ) Light Chain-containing Human (IgM) Antibodies: Polysialic Acid (PSA) Attached to NCAM as a Case Study

DOI:
10.3791/54139-v

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11:10 min

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June 29, 2016

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, , , , , ,
1Department of Neurology,Mayo Klinik, 2Mayo Clinic Center for Multiple Sclerosis and Autoimmune Neurology,Mayo Klinik, 3Center for Regenerative Medicine, Neuroregeneration,Mayo Klinik, 4Division of Neonatal Medicine,Mayo Klinik, 5Department of Pediatric and Adolescent Medicine,Mayo Klinik

Bölümler

  • 00:05Başlık
  • 01:09General CNS Tissue Preparation
  • 02:42Protein L Agarose Bead Preparation
  • 03:43Antibody-Antigen-Agarose L Complex Formation and Antigen Detection in Western Blots
  • 08:44Results: Antibody Binding Specificity for Kappa Light Chain-containing Human Antibodies
  • 09:54Conclusion

Özet

Otomatik Çeviri

Otomatik Çeviri

We present a protocol to identify protein moieties containing antigens for human and mouse IgM antibodies with specific kappa (Vκ) light chains. This protocol is not limited to IgM class antibodies but applies to all immunoglobulin isotypes that target their antigen with sufficiently high affinity during immunoprecipitations.

Etiketler

Antibody BindingKappa Light ChainIgM AntibodiesPolysialic AcidNCAMAntigen IdentificationTherapeutic PotentialIgM IsotypeBiomarkersImmunotherapeutic AgentsKanser AraştırmalarıCentral Nervous SystemLysateImmunoprecipitation

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