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Herstellung von Einzelzellsuspensionen für Cytofluorimetric Analyse von verschiedenen Mäusehaut Regionen
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Herstellung von Einzelzellsuspensionen für Cytofluorimetric Analyse von verschiedenen Mäusehaut Regionen
Bölümler
- 00:05Başlık
- 00:44Skin Sample Collection
- 02:29Skin Sample Immunostaining
- 04:11Flow Cytometric Analysis
- 04:59Results: Quantitative Analysis of Mouse Immune Cells from Different Regions of the Skin
- 05:32Conclusion
The skin is home to a complex immune cell network. We describe an efficient methodology for the digestion of mouse skin, from different parts of the animal's body, in order to obtain a single-cell suspension and analyze the different leukocyte populations resident in the skin by flow cytometry.
