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Préparation de Suspensions de cellules uniques pour l'analyse cytofluorimétrie de différentes régions de la peau de souris
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Préparation de Suspensions de cellules uniques pour l'analyse cytofluorimétrie de différentes régions de la peau de souris
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April 20, 2016
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The skin is home to a complex immune cell network. We describe an efficient methodology for the digestion of mouse skin, from different parts of the animal's body, in order to obtain a single-cell suspension and analyze the different leukocyte populations resident in the skin by flow cytometry.
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Broggi, A., Cigni, C., Zanoni, I., Granucci, F. Preparation of Single-cell Suspensions for Cytofluorimetric Analysis from Different Mouse Skin Regions. J. Vis. Exp. (110), e52589, doi:10.3791/52589 (2016).
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