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单细胞悬液的细胞荧光分析从不同的鼠标皮肤区域准备
JoVE Journal
İmmünoloji ve Enfeksiyon
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JoVE Journal İmmünoloji ve Enfeksiyon
Preparation of Single-cell Suspensions for Cytofluorimetric Analysis from Different Mouse Skin Regions

单细胞悬液的细胞荧光分析从不同的鼠标皮肤区域准备

17,585 Views

05:58 min

April 20, 2016

DOI:

05:58 min
April 20, 2016

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Özet

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The skin is home to a complex immune cell network. We describe an efficient methodology for the digestion of mouse skin, from different parts of the animal's body, in order to obtain a single-cell suspension and analyze the different leukocyte populations resident in the skin by flow cytometry.

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