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Preparação de uma única célula suspensões para Análise de citometria de fluxo de diferentes regiões da pele do rato
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JoVE Journal İmmünoloji ve Enfeksiyon
Preparation of Single-cell Suspensions for Cytofluorimetric Analysis from Different Mouse Skin Regions

Preparação de uma única célula suspensões para Análise de citometria de fluxo de diferentes regiões da pele do rato

17,585 Views

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05:58 min

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April 20, 2016

DOI:

10.3791/52589-v

DOI:
10.3791/52589-v

•

05:58 min
April 20, 2016

•

30 Views
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1Department of Biotechnology and Biosciences,University of Milano-Bicocca, 2Boston Children’s Hospital, Division of Gastroenterology,Harvard Tıp Fakültesi, 3Humanitas Clinical and Research Center

Özet

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The skin is home to a complex immune cell network. We describe an efficient methodology for the digestion of mouse skin, from different parts of the animal's body, in order to obtain a single-cell suspension and analyze the different leukocyte populations resident in the skin by flow cytometry.

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Single-cell SuspensionCytofluorimetric AnalysisMouse SkinResident Immune CellsSkin SamplingSkin DigestionSkin Cell Labeling

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Broggi, A., Cigni, C., Zanoni, I., Granucci, F. Preparation of Single-cell Suspensions for Cytofluorimetric Analysis from Different Mouse Skin Regions. J. Vis. Exp. (110), e52589, doi:10.3791/52589 (2016).

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