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Preparation of Single-cell Suspensions for Cytofluorimetric Analysis from Different Mouse Skin Regions
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Preparation of Single-cell Suspensions for Cytofluorimetric Analysis from Different Mouse Skin Regions

Preparation of Single-cell Suspensions for Cytofluorimetric Analysis from Different Mouse Skin Regions

17,605 Views

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05:58 min

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April 20, 2016

DOI:

10.3791/52589-v

DOI:
10.3791/52589-v

•

05:58 min
April 20, 2016

•

30 Views
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1Department of Biotechnology and Biosciences,University of Milano-Bicocca, 2Boston Children’s Hospital, Division of Gastroenterology,Harvard Medical School, 3Humanitas Clinical and Research Center

Summary

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The skin is home to a complex immune cell network. We describe an efficient methodology for the digestion of mouse skin, from different parts of the animal's body, in order to obtain a single-cell suspension and analyze the different leukocyte populations resident in the skin by flow cytometry.

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Single-cell SuspensionCytofluorimetric AnalysisMouse SkinResident Immune CellsSkin SamplingSkin DigestionSkin Cell Labeling

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Broggi, A., Cigni, C., Zanoni, I., Granucci, F. Preparation of Single-cell Suspensions for Cytofluorimetric Analysis from Different Mouse Skin Regions. J. Vis. Exp. (110), e52589, doi:10.3791/52589 (2016).

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