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内吞体动力学在生活神经末梢与四维荧光成像可视化
JoVE Journal
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JoVE Journal Nörobilim
Visualization of Endosome Dynamics in Living Nerve Terminals with Four-dimensional Fluorescence Imaging
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内吞体动力学在生活神经末梢与四维荧光成像可视化

DOI:
10.3791/51477-v

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10:51 min

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April 16, 2014

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, ,
1Department of Cell Biology and Physiology,Washington Üniversitesi Tıp Fakültesi

'Bölümler'

  • 00:05'Başlık'
  • 01:10Staining the Preparation with Supravital Dyes
  • 02:30Configuring the Preparation for Imaging
  • 03:31Setting Parameters for 4D Imaging
  • 04:52Data Analysis
  • 06:54Results: 4D Live Imaging Reveals Dynamic Behavior of Macroendosomes and Acidic Endosomes in Nerve Terminals
  • 10:23Conclusion

Özet

'Otomatik Çeviri'

'Otomatik Çeviri'

四维（4D）成像被用于研究中两种类型的核内体的活的脊椎动物神经终端的行为和交互作用。这些小的结构的运动的特征是在三维空间中，从而允许确认如内涵体融合和胞吐作用的事件。

'Etiketler'

4D Fluorescence ImagingEndosome DynamicsNerve TerminalsMacroendosomesAcidic Endosomes3D Time-lapseDeconvolutionQuantitative Analysis

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