Rivista
/
Neuroscienze
/
Visualization of Endosome Dynamics in Living Nerve Terminals with Four-dimensional Fluorescence Imaging
JoVE Journal
Neuroscienze
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Neuroscienze
Visualization of Endosome Dynamics in Living Nerve Terminals with Four-dimensional Fluorescence Imaging

Visualization of Endosome Dynamics in Living Nerve Terminals with Four-dimensional Fluorescence Imaging

DOI:
10.3791/51477-v

•

10:51 min

•

April 16, 2014

•

, ,
1Department of Cell Biology and Physiology,Washington University School of Medicine

Capitoli

  • 00:05Titolo
  • 01:10Staining the Preparation with Supravital Dyes
  • 02:30Configuring the Preparation for Imaging
  • 03:31Setting Parameters for 4D Imaging
  • 04:52Data Analysis
  • 06:54Results: 4D Live Imaging Reveals Dynamic Behavior of Macroendosomes and Acidic Endosomes in Nerve Terminals
  • 10:23Conclusion

Summary

Traduzione automatica

Traduzione automatica

Four-dimensional (4D) imaging is utilized to study the behavior and interactions among two types of endosomes in living vertebrate nerve terminals. Movement of these small structures is characterized in three dimensions, permitting confirmation of events such as endosome fusion and exocytosis.

Tags

Keywords: 4D Fluorescence ImagingEndosome DynamicsNerve TerminalsMacroendosomesAcidic Endosomes3D Time-lapseDeconvolutionQuantitative Analysis

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/51477?article_type=v

Preparation of Living Isolated Vertebrate Photoreceptor Cells for Fluorescence Imaging
check_url/it/51477?article_type=v

Patch-clamp Capacitance Measurements and Ca2+ Imaging at Single Nerve Terminals in Retinal Slices
check_url/it/51477?article_type=v

Visualization of Proprioceptors in Drosophila Larvae and Pupae
check_url/it/51477?article_type=v

Deep Brain Stimulation with Simultaneous fMRI in Rodents
check_url/it/51477?article_type=v

Two-photon Imaging of Cellular Dynamics in the Mouse Spinal Cord
check_url/it/51477?article_type=v

Imaging Ca2+ Dynamics in Cone Photoreceptor Axon Terminals of the Mouse Retina
check_url/it/51477?article_type=v

Optical Monitoring of Living Nerve Terminal Labeling in Hair Follicle Lanceolate Endings of the Ex Vivo Mouse Ear Skin
check_url/it/51477?article_type=v

Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes
check_url/it/51477?article_type=v

Visualization of Amyloid β Deposits in the Human Brain with Matrix-assisted Laser Desorption/Ionization Imaging Mass Spectrometry
check_url/it/51477?article_type=v

Preparation of Newborn Rat Brain Tissue for Ultrastructural Morphometric Analysis of Synaptic Vesicle Distribution at Nerve Terminals

Read Article