'Dergi'
/
Nörobilim
/
Visualization of Endosome Dynamics in Living Nerve Terminals with Four-dimensional Fluorescence Imaging
JoVE Journal
Nörobilim
'Bu içeriği görüntülemek için JoVE aboneliği gereklidir.'  'Oturum açın veya ücretsiz deneme sürümünü başlatın.'
JoVE Journal Nörobilim
Visualization of Endosome Dynamics in Living Nerve Terminals with Four-dimensional Fluorescence Imaging

Visualization of Endosome Dynamics in Living Nerve Terminals with Four-dimensional Fluorescence Imaging

DOI:
10.3791/51477-v

•

10:51 min

•

April 16, 2014

•

, ,
1Department of Cell Biology and Physiology,Washington Üniversitesi Tıp Fakültesi

'Bölümler'

  • 00:05'Başlık'
  • 01:10Staining the Preparation with Supravital Dyes
  • 02:30Configuring the Preparation for Imaging
  • 03:31Setting Parameters for 4D Imaging
  • 04:52Data Analysis
  • 06:54Results: 4D Live Imaging Reveals Dynamic Behavior of Macroendosomes and Acidic Endosomes in Nerve Terminals
  • 10:23Conclusion

Özet

'Otomatik Çeviri'

'Otomatik Çeviri'

Four-dimensional (4D) imaging is utilized to study the behavior and interactions among two types of endosomes in living vertebrate nerve terminals. Movement of these small structures is characterized in three dimensions, permitting confirmation of events such as endosome fusion and exocytosis.

'Etiketler'

4D Fluorescence ImagingEndosome DynamicsNerve TerminalsMacroendosomesAcidic Endosomes3D Time-lapseDeconvolutionQuantitative Analysis

Article

Embed

'ÇALMA LİSTESİNE EKLE'

Usage Statistics

'İlgili Videolar'

Preparation of Living Isolated Vertebrate Photoreceptor Cells for Fluorescence Imaging

Patch-clamp Capacitance Measurements and Ca2+ Imaging at Single Nerve Terminals in Retinal Slices

Visualization of Proprioceptors in Drosophila Larvae and Pupae

Deep Brain Stimulation with Simultaneous fMRI in Rodents

Two-photon Imaging of Cellular Dynamics in the Mouse Spinal Cord

Imaging Ca2+ Dynamics in Cone Photoreceptor Axon Terminals of the Mouse Retina

Optical Monitoring of Living Nerve Terminal Labeling in Hair Follicle Lanceolate Endings of the Ex Vivo Mouse Ear Skin

Quantification of Endosome and Lysosome Motilities in Cultured Neurons Using Fluorescent Probes

Visualization of Amyloid β Deposits in the Human Brain with Matrix-assisted Laser Desorption/Ionization Imaging Mass Spectrometry

Preparation of Newborn Rat Brain Tissue for Ultrastructural Morphometric Analysis of Synaptic Vesicle Distribution at Nerve Terminals

Read Article