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Visualization of Endosome Dynamics in Living Nerve Terminals with Four-dimensional Fluorescence Imaging
JoVE Journal
神経科学
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JoVE Journal 神経科学
Visualization of Endosome Dynamics in Living Nerve Terminals with Four-dimensional Fluorescence Imaging
DOI:

10:51 min

April 16, 2014

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  • 00:05標題
  • 01:10Staining the Preparation with Supravital Dyes
  • 02:30Configuring the Preparation for Imaging
  • 03:31Setting Parameters for 4D Imaging
  • 04:52Data Analysis
  • 06:54Results: 4D Live Imaging Reveals Dynamic Behavior of Macroendosomes and Acidic Endosomes in Nerve Terminals
  • 10:23Conclusion

概要

自動翻訳

Four-dimensional (4D) imaging is utilized to study the behavior and interactions among two types of endosomes in living vertebrate nerve terminals. Movement of these small structures is characterized in three dimensions, permitting confirmation of events such as endosome fusion and exocytosis.

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