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Visualization of Endosome Dynamics in Living Nerve Terminals with Four-dimensional Fluorescence Imaging
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JoVE Journal Neuroscience
Visualization of Endosome Dynamics in Living Nerve Terminals with Four-dimensional Fluorescence Imaging

Visualization of Endosome Dynamics in Living Nerve Terminals with Four-dimensional Fluorescence Imaging

DOI:
10.3791/51477-v

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10:51 min

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April 16, 2014

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, ,
1Department of Cell Biology and Physiology,Washington University School of Medicine

Chapters

  • 00:05Title
  • 01:10Staining the Preparation with Supravital Dyes
  • 02:30Configuring the Preparation for Imaging
  • 03:31Setting Parameters for 4D Imaging
  • 04:52Data Analysis
  • 06:54Results: 4D Live Imaging Reveals Dynamic Behavior of Macroendosomes and Acidic Endosomes in Nerve Terminals
  • 10:23Conclusion

Summary

Automatic Translation

Automatic Translation

Four-dimensional (4D) imaging is utilized to study the behavior and interactions among two types of endosomes in living vertebrate nerve terminals. Movement of these small structures is characterized in three dimensions, permitting confirmation of events such as endosome fusion and exocytosis.

Tags

Keywords: 4D Fluorescence ImagingEndosome DynamicsNerve TerminalsMacroendosomesAcidic Endosomes3D Time-lapseDeconvolutionQuantitative Analysis

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