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Neuroscience

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JoVE Journal Neuroscience
Easy and Reproducible Low-Density Primary Culture using Frozen Stock of Embryonic Hippocampal Neurons
 

Easy and Reproducible Low-Density Primary Culture using Frozen Stock of Embryonic Hippocampal Neurons

Article DOI: 10.3791/64872-v • 04:26 min • January 27th, 2023
January 27th, 2023 •
1, 2, 3,4, 3, 5, 1,5
1Department of Pharmacology, Graduate School of Medicine, Gunma University, 2Gunma University Initiative for Advanced Research, Gunma University, 3Department of Veterinary Pathophysiology and Animal Health, Graduate school of Agricultural and Life Sciences, The University of Tokyo, 4Institute for Drug Discovery Innovation, 5AlzMed, Inc.

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A ready-to-use frozen stock of neurons is a powerful tool for evaluating synaptic functions. Here, we introduce an easy low-density primary culture from frozen stock using a 96-well plate.

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Low-density Primary Culture Frozen Stock Embryonic Hippocampal Neurons Culturing Cells Easy Technique Reproducible Technique No Advanced Techniques Required Animal Experimentation Timed Pregnant Animals Dissecting Embryos Other Culture Vessels Microelectrode Array Plates Electrophysiological Experiments Poly-L-lysine Coating Sterilized Water Culture Medium Cell Seeding Neuron Cryo Vial
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