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배아 해마 뉴런의 냉동 재고를 사용한 쉽고 재현 가능한 저밀도 1차 배양
JoVE Journal
Neurociência
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JoVE Journal Neurociência
Easy and Reproducible Low-Density Primary Culture using Frozen Stock of Embryonic Hippocampal Neurons
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배아 해마 뉴런의 냉동 재고를 사용한 쉽고 재현 가능한 저밀도 1차 배양

1,911 Views

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04:26 min

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January 27, 2023

DOI:

10.3791/64872-v

DOI:
10.3791/64872-v

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04:26 min
January 27, 2023

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4 Views
, , , , ,
1Department of Pharmacology, Graduate School of Medicine,Gunma University, 2Gunma University Initiative for Advanced Research,Gunma University, 3Department of Veterinary Pathophysiology and Animal Health, Graduate school of Agricultural and Life Sciences,The University of Tokyo, 4Institute for Drug Discovery Innovation, 5AlzMed, Inc.

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Low-density Primary CultureFrozen StockEmbryonic Hippocampal NeuronsCell CulturePoly-L-lysineMicroelectrode ArrayCell SeedingCell SuspensionCytosine Beta-D-arabinofuranosideGlial Cell Growth

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Koganezawa, N., Roppongi, R. T., Sekino, Y., Tsutsui, I., Higa, A., Shirao, T. Easy and Reproducible Low-Density Primary Culture using Frozen Stock of Embryonic Hippocampal Neurons. J. Vis. Exp. (191), e64872, doi:10.3791/64872 (2023).

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