Conducting in vitro experiments to reflect in vivo conditions as adequately as possible is not an easy task. The use of primary cell cultures is an important step toward understanding cell biology in a whole organism. The provided protocol outlines how to successfully grow and culture embryonic mouse cerebellar neurons.
Shabanipour, S., Dalvand, A., Jiao, X., Rahimi Balaei, M., Chung, S. H., Kong, J., Del Bigio, M. R., Marzban, H. Primary Culture of Neurons Isolated from Embryonic Mouse Cerebellum. J. Vis. Exp. (152), e60168, doi:10.3791/60168 (2019).