É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Low-Density Primary Hippocampal Neuron Culture
20,083 Views
•
17:46 min
•
April 18, 2017
DOI:
Summary
Automatically generated
This article describes the protocol for culturing low-density primary hippocampal neurons growing on glass coverslips inverted over a glial monolayer. The neuron and glial layers are separated by paraffin wax beads. The neurons grown by this method are suitable for high-resolution optical imaging and functional assays.
Read Article
Cite this Article
Roppongi, R. T., Champagne-Jorgensen, K. P., Siddiqui, T. J. Low-Density Primary Hippocampal Neuron Culture. J. Vis. Exp. (122), e55000, doi:10.3791/55000 (2017).
Copy