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Primary Culture of Mouse Dopaminergic Neurons
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Neurociência
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JoVE Journal Neurociência
Primary Culture of Mouse Dopaminergic Neurons

Primary Culture of Mouse Dopaminergic Neurons

36,757 Views

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11:58 min

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September 08, 2014

DOI:

10.3791/51751-v

DOI:
10.3791/51751-v

•

11:58 min
September 08, 2014

•

43 Views
, ,
1CNRS, UMR-5203,Institut de Génomique Fonctionnelle, Montpellier, 2Inserm,U661, Montpellier, 3Universités de Montpellier

Summary

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Dopaminergic neurons play a vital regulatory role in the brain. Their loss is associated with Parkinson's disease. In this video, we show how to generate primary cultures of central dopaminergic neurons from embryonic mouse mesencephalon. Such cultures are useful to study the extreme vulnerability of these neurons to various stresses.

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Cite this Article

Gaven, F., Marin, P., Claeysen, S. Primary Culture of Mouse Dopaminergic Neurons. J. Vis. Exp. (91), e51751, doi:10.3791/51751 (2014).

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