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Coltura primaria a bassa densità facile e riproducibile utilizzando stock congelati di neuroni ippocampali embrionali

JoVE Journal
Neurociência

This content is Free Access.

JoVE Journal Neurociência

Easy and Reproducible Low-Density Primary Culture using Frozen Stock of Embryonic Hippocampal Neurons

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Coltura primaria a bassa densità facile e riproducibile utilizzando stock congelati di neuroni ippocampali embrionali

1,911 Views

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04:26 min

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January 27, 2023

DOI:

10.3791/64872-v

DOI:

10.3791/64872-v

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04:26 min

January 27, 2023

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4 Views

Noriko Koganezawa, Reiko T. Roppongi, Yuko Sekino⁴, Izuo Tsutsui, Ayaka Higa, Tomoaki Shirao⁵

¹Department of Pharmacology, Graduate School of Medicine,Gunma University, ²Gunma University Initiative for Advanced Research,Gunma University, ³Department of Veterinary Pathophysiology and Animal Health, Graduate school of Agricultural and Life Sciences,The University of Tokyo, ⁴Institute for Drug Discovery Innovation, ⁵AlzMed, Inc.

Tags

Low-density Primary Culture Frozen Stock Embryonic Hippocampal Neurons Cell Culture Poly-L-lysine Microelectrode Array Cell Seeding Cell Suspension Cytosine Beta-D-arabinofuranoside Glial Cell Growth

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