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使用二分法减少牛卵母细胞中的线粒体DNA
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Use of Bisection to Reduce Mitochondrial DNA in the Bovine Oocyte
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使用二分法减少牛卵母细胞中的线粒体DNA

DOI:
10.3791/64060-v

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06:15 min

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July 06, 2022

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, , , , , ,
1Department of Animal, Dairy, and Veterinary Sciences,Utah State University, 2Reproductive Sciences, Beckman Center for Conservation Research,San Diego Zoo Wildlife Alliance, 3Genus PLC

Capítulos

  • 00:04Introduction
  • 00:47Centrifugation of the Oocytes
  • 01:51Preparation of the Oocyte for Bisection
  • 03:00Bisection of the Oocytes
  • 04:56Results: Successful and Unsuccessful qPCR Results and Boxplot from Mitochondria-Reduced and Mitochondria-Dense Ooplasts
  • 05:39Conclusion

Summary

Tadução automática

Tadução automática

在这里，我们提出了一种方案，以显着减少牛卵母细胞中的线粒体DNA拷贝数（P <0.0001）。该方法利用离心和二分切来大大减少卵母细胞线粒体，并且可以增加在重建的种间体细胞核移植胚胎中发育的机会。

Tags

BisectionMitochondrial DNABovine OocyteCloningHeteroplasmyCentrifugationZonae PellucidaePronaseCBT20

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