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从产甲烷纯培养物和环境样品中提取辅因子_F420 用于分析聚谷氨酸尾部长度

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Extraction of Cofactor F₄₂₀ for Analysis of Polyglutamate Tail Length from Methanogenic Pure Cultures and Environmental Samples

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从产甲烷纯培养物和环境样品中提取辅因子_F420 用于分析聚谷氨酸尾部长度

DOI:

10.3791/62737-v

•

04:32 min

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October 14, 2021

•

Rudolf Markt, Mathias Wunderer, Eva Maria Prem, Mira Mutschlechner, Nina Lackner, Andreas Otto Wagner

¹Department of Microbiology,Universität Innsbruck, ²Department of Hygiene and Medical Microbiology,Medical University of Innsbruck

Capítulos

00:05Introduction
00:49Sample Lysis and Pre-Purification of the Cofactor F₄₂₀ via Solid-Phase Extraction (SPE)
02:18Detection of the Cofactor F₄₂₀
03:02Results: Different F₄₂₀ Tail-Length Profiles for Methanogens
03:56Conclusion

Summary

Tadução automática

针对纯培养物和环境样品中_F420 尾长的液相色谱分离和分析，优化了从纯培养物中提取辅因子_F420 的方法。

Tags

Keywords: Cofactor F420 Methanogenic Polyglutamate Environmental Samples Sample Lysis Solid-phase Extraction HPLC Analysis

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