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研究斑马鱼脊椎动物分割的三维尾部外植培养
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
A 3-D Tail Explant Culture to Study Vertebrate Segmentation in Zebrafish
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研究斑马鱼脊椎动物分割的三维尾部外植培养

DOI:
10.3791/61981-v

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11:24 min

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June 30, 2021

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1Division of Developmental Biology,Cincinnati Children’s Hospital Medical Center, 2Department of Pediatrics,University of Cincinnati College of Medicine, 3Department of Genetics,Albert Einstein College of Medicine

Capítulos

  • 00:00Introduction
  • 00:21Zebrafish Embryo Collection
  • 01:50Tools Preparation
  • 03:33Media Recipes
  • 05:183-D Tail Explants
  • 08:42Confocal Microscopy with 3-D Explants
  • 10:30Representative Results
  • 10:56Conclusion

Summary

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在这里，我们介绍了斑马鱼后体轴的三维组织培养方案，从而能够对脊椎动物分割进行实时研究。此除草模型可控制轴伸长、形态源的改变以及细胞下分辨率组织水平的实时成像。

Tags

3-D Tail ExplantZebrafishVertebrate SegmentationSomite DevelopmentPresomitic MesodermEmbryo TransparencyTime-lapse ImagingDissection TechniquesVertebrate Patterning

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