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A 3-D Tail Explant Culture to Study Vertebrate Segmentation in Zebrafish
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Developmental Biology
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JoVE Journal Developmental Biology
A 3-D Tail Explant Culture to Study Vertebrate Segmentation in Zebrafish

A 3-D Tail Explant Culture to Study Vertebrate Segmentation in Zebrafish

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3,971 Views

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11:24 min

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June 30, 2021

DOI:

10.3791/61981-v

DOI:
10.3791/61981-v

•

11:24 min
June 30, 2021

•

1 Views
,
1Division of Developmental Biology,Cincinnati Children’s Hospital Medical Center, 2Department of Pediatrics,University of Cincinnati College of Medicine, 3Department of Genetics,Albert Einstein College of Medicine

Summary

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Here, we present the protocol for 3-D tissue culture of the zebrafish posterior body axis, enabling live study of vertebrate segmentation. This explant model provides control over axis elongation, alteration of morphogen sources, and subcellular resolution tissue-level live imaging.

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Cite this Article

Simsek, M. F., Özbudak, E. M. A 3-D Tail Explant Culture to Study Vertebrate Segmentation in Zebrafish. J. Vis. Exp. (172), e61981, doi:10.3791/61981 (2021).

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