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A 3-D Tail Explant Culture to Study Vertebrate Segmentation in Zebrafish
JoVE Journal
Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
A 3-D Tail Explant Culture to Study Vertebrate Segmentation in Zebrafish

A 3-D Tail Explant Culture to Study Vertebrate Segmentation in Zebrafish

3,980 Views

11:24 min

June 30, 2021

DOI:

11:24 min
June 30, 2021

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Summary

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Here, we present the protocol for 3-D tissue culture of the zebrafish posterior body axis, enabling live study of vertebrate segmentation. This explant model provides control over axis elongation, alteration of morphogen sources, and subcellular resolution tissue-level live imaging.

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