Journal
/
Biologie du développement
/
A 3-D Tail Explant Culture to Study Vertebrate Segmentation in Zebrafish
Journal JoVE
Biologie du développement
Author Produced
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie du développement
A 3-D Tail Explant Culture to Study Vertebrate Segmentation in Zebrafish

A 3-D Tail Explant Culture to Study Vertebrate Segmentation in Zebrafish

3,971 Views

•

11:24 min

•

June 30, 2021

DOI:

10.3791/61981-v

DOI:
10.3791/61981-v

•

11:24 min
June 30, 2021

•

1 Views
,
1Division of Developmental Biology,Cincinnati Children’s Hospital Medical Center, 2Department of Pediatrics,University of Cincinnati College of Medicine, 3Department of Genetics,Albert Einstein College of Medicine

Summary

Automatically generated

Here, we present the protocol for 3-D tissue culture of the zebrafish posterior body axis, enabling live study of vertebrate segmentation. This explant model provides control over axis elongation, alteration of morphogen sources, and subcellular resolution tissue-level live imaging.

Explore More Videos

3-D Tail ExplantZebrafishVertebrate SegmentationSomite DevelopmentPresomitic MesodermEmbryo TransparencyTime-lapse ImagingDissection TechniquesVertebrate Patterning

Read Article

Cite this Article

Simsek, M. F., Özbudak, E. M. A 3-D Tail Explant Culture to Study Vertebrate Segmentation in Zebrafish. J. Vis. Exp. (172), e61981, doi:10.3791/61981 (2021).

Download .ris file

Copy

Share Video

.

Copy