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Een 3D Tail Explant-cultuur om gewervelde segmentatie bij zebravissen te bestuderen
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
A 3-D Tail Explant Culture to Study Vertebrate Segmentation in Zebrafish
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Een 3D Tail Explant-cultuur om gewervelde segmentatie bij zebravissen te bestuderen

3,971 Views

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11:24 min

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June 30, 2021

DOI:

10.3791/61981-v

DOI:
10.3791/61981-v

•

11:24 min
June 30, 2021

•

1 Views
,
1Division of Developmental Biology,Cincinnati Children’s Hospital Medical Center, 2Department of Pediatrics,University of Cincinnati College of Medicine, 3Department of Genetics,Albert Einstein College of Medicine

Summary

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Hier presenteren we het protocol voor 3D-weefselkweek van de achterste lichaamsas van zebravissen, waardoor live studie van gewervelde segmentatie mogelijk is. Dit explantmodel biedt controle over de verlenging van de as, wijziging van morfogene bronnen en live beeldvorming op weefselniveau op subcellulair resolutieniveau.

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Cite this Article

Simsek, M. F., Özbudak, E. M. A 3-D Tail Explant Culture to Study Vertebrate Segmentation in Zebrafish. J. Vis. Exp. (172), e61981, doi:10.3791/61981 (2021).

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