Disruption of the blood-spinal cord barrier (BSCB) can be successfully achieved with the intravenous administration of microbubbles and the application of low-intensity focused ultrasound (LIFU). This protocol details the opening of the BSCB using LIFU in a rodent model, including equipment setup, microbubble injection, target localization, and BSCB disruption visualization.
Bhimreddy, M., Routkevitch, D., Hersh, A. M., Mohammadabadi, A., Menta, A. K., Jiang, K., Weber-Levine, C., Davidar, A. D., Punnoose, J., Kempski Leadingham, K. M., Doloff, J. C., Tyler, B., Theodore, N., Manbachi, A. Disruption of the Blood-Spinal Cord Barrier Using Low-Intensity Focused Ultrasound in a Rat Model. J. Vis. Exp. (193), e65113, doi:10.3791/65113 (2023).