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Disruption of the Blood-Spinal Cord Barrier Using Low-Intensity Focused Ultrasound in a Rat Model
JoVE Journal
Bioengenharia
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JoVE Journal Bioengenharia
Disruption of the Blood-Spinal Cord Barrier Using Low-Intensity Focused Ultrasound in a Rat Model

Disruption of the Blood-Spinal Cord Barrier Using Low-Intensity Focused Ultrasound in a Rat Model

1,514 Views

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09:03 min

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March 10, 2023

DOI:

10.3791/65113-v

DOI:
10.3791/65113-v

•

09:03 min
March 10, 2023

•

7 Views
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1Department of Neurosurgery,Johns Hopkins University School of Medicine, 2Department of Biomedical Engineering,Johns Hopkins University, 3HEPIUS Innovation Laboratory,Johns Hopkins University School of Medicine, 4Department of Electrical Engineering and Computer Science,Johns Hopkins University, 5Department of Mechanical Engineering,Johns Hopkins University, 6Department of Anesthesiology and Critical Care Medicine,Johns Hopkins University

Summary

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Disruption of the blood-spinal cord barrier (BSCB) can be successfully achieved with the intravenous administration of microbubbles and the application of low-intensity focused ultrasound (LIFU). This protocol details the opening of the BSCB using LIFU in a rodent model, including equipment setup, microbubble injection, target localization, and BSCB disruption visualization.

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Cite this Article

Bhimreddy, M., Routkevitch, D., Hersh, A. M., Mohammadabadi, A., Menta, A. K., Jiang, K., Weber-Levine, C., Davidar, A. D., Punnoose, J., Kempski Leadingham, K. M., Doloff, J. C., Tyler, B., Theodore, N., Manbachi, A. Disruption of the Blood-Spinal Cord Barrier Using Low-Intensity Focused Ultrasound in a Rat Model. J. Vis. Exp. (193), e65113, doi:10.3791/65113 (2023).

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