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Disruption of the Blood-Spinal Cord Barrier Using Low-Intensity Focused Ultrasound in a Rat Model
This content is Free Access.
1Department of Neurosurgery,Johns Hopkins University School of Medicine, 2Department of Biomedical Engineering,Johns Hopkins University, 3HEPIUS Innovation Laboratory,Johns Hopkins University School of Medicine, 4Department of Electrical Engineering and Computer Science,Johns Hopkins University, 5Department of Mechanical Engineering,Johns Hopkins University, 6Department of Anesthesiology and Critical Care Medicine,Johns Hopkins University
Chapters
- 00:04Introduction
- 01:11Low‐Intensity Focused Ultrasound Assembly and Setup
- 02:20Animal Preparation and Surgical Laminectomy
- 03:38Target Localization Using Laser Guidance
- 04:22Microbubble Administration
- 05:27Spinal Cord Extraction, Tissue Processing, and Visualization of BSCB Disruption
- 06:34Results: Confirmation of Target Localization and Evaluating BSCB Disruption by EBD Extravasation into the Spinal Cord
- 08:17Conclusion
Disruption of the blood-spinal cord barrier (BSCB) can be successfully achieved with the intravenous administration of microbubbles and the application of low-intensity focused ultrasound (LIFU). This protocol details the opening of the BSCB using LIFU in a rodent model, including equipment setup, microbubble injection, target localization, and BSCB disruption visualization.
