Journal
/
면역학 및 감염병학
/
Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils
JoVE 신문
면역학 및 감염병학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 면역학 및 감염병학
Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils

Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils

1,386 Views

•

09:39 min

•

June 02, 2023

DOI:

10.3791/64971-v

DOI:
10.3791/64971-v

•

09:39 min
June 02, 2023

•

16 Views
, ,
1Department of Immunology, School of Medicine,UConn Health, 2Arthritis and Clinical Immunology Program,Oklahoma Medical Research Foundation, 3Department of Microbiology and Immunology,University of Oklahoma Health Science Center

Summary

Automatically generated

We describe stepwise protocols measuring the mitochondrial respiration of mouse and human neutrophils and HL60 cells using the metabolic extracellular flux analyzer.

Explore More Videos

Mitochondrial RespirationNeutrophilsHL60 CellsMetabolic Extracellular Flux AnalyzerMitochondrial FunctionCell Seeding DensityAssay MediumMouse NeutrophilsHuman NeutrophilsDifferentiated HL60 CellsUndifferentiated HL60 CellsBackground Correction

Read Article

Cite this Article

Pulikkot, S., Zhao, M., Fan, Z. Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils. J. Vis. Exp. (196), e64971, doi:10.3791/64971 (2023).

Download .ris file

Copy

Share Video

.

Copy