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Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils
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Inmunología y contagio
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JoVE Revista Inmunología y contagio
Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils

Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils

1,386 Views

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09:39 min

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June 02, 2023

DOI:

10.3791/64971-v

DOI:
10.3791/64971-v

•

09:39 min
June 02, 2023

•

16 Views
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1Department of Immunology, School of Medicine,UConn Health, 2Arthritis and Clinical Immunology Program,Oklahoma Medical Research Foundation, 3Department of Microbiology and Immunology,University of Oklahoma Health Science Center

Summary

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We describe stepwise protocols measuring the mitochondrial respiration of mouse and human neutrophils and HL60 cells using the metabolic extracellular flux analyzer.

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Mitochondrial RespirationNeutrophilsHL60 CellsMetabolic Extracellular Flux AnalyzerMitochondrial FunctionCell Seeding DensityAssay MediumMouse NeutrophilsHuman NeutrophilsDifferentiated HL60 CellsUndifferentiated HL60 CellsBackground Correction

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Cite this Article

Pulikkot, S., Zhao, M., Fan, Z. Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils. J. Vis. Exp. (196), e64971, doi:10.3791/64971 (2023).

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