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Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils
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JoVE Journal 免疫学と感染
Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils

Real-Time Measurement of the Mitochondrial Bioenergetic Profile of Neutrophils

DOI:
10.3791/64971-v

•

09:39 min

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June 02, 2023

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, ,
1Department of Immunology, School of Medicine,UConn Health, 2Arthritis and Clinical Immunology Program,Oklahoma Medical Research Foundation, 3Department of Microbiology and Immunology,University of Oklahoma Health Science Center

章

  • 00:05Introduction
  • 01:05Preparation of the 96-Well Plate for the Metabolic Extracellular Flux Assay
  • 01:52Preparation and Seeding of Cells
  • 03:52Preparing Compounds in the Mitochondrial Stress Test Kit
  • 04:25Running the Mitochondrial Stress Assay
  • 07:29Results: Metabolic Flux Assay to Analyze Oxygen Consumption Rates (OCR) in Neutrophils
  • 09:02Conclusion

概要

自動翻訳

自動翻訳

We describe stepwise protocols measuring the mitochondrial respiration of mouse and human neutrophils and HL60 cells using the metabolic extracellular flux analyzer.

タグ

Mitochondrial RespirationNeutrophilsHL60 CellsMetabolic Extracellular Flux AnalyzerMitochondrial FunctionCell Seeding DensityAssay MediumMouse NeutrophilsHuman NeutrophilsDifferentiated HL60 CellsUndifferentiated HL60 CellsBackground Correction

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