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Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells
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면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

DOI:
10.3791/64918-v

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10:20 min

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March 24, 2023

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, , , , ,
1Quantitative Immuno-hematology, CNRS UMR168,Institut Curie, 2Hamilton Institute,Maynooth University, 3Institut Curie, PSL Research University, CNRS UMR 3348, Orsay, 4Université Paris-Sud, Université Paris-Saclay, CNRS UMR 3348, Orsay

Chapters

  • 00:04Introduction
  • 00:51Cell Dye Staining
  • 02:33Antibody Staining and Cell Sorting
  • 05:41After Culture Antibody Staining
  • 06:47Post‐Culture Flow Cytometry Data Analysis
  • 08:01Results: Simultaneous Assessment of Cell Division Number, Surface Cell Phenotype, and Cellular Kinship
  • 09:26Conclusion

Summary

자동 번역

자동 번역

Presented here is a flow cytometry-based technique that allows for simultaneously measuring the number of cell divisions, surface cell phenotype, and cellular kinship. Those properties can be tested statistically using a permutation-based framework.

Tags

Hematopoietic Stem CellsHematopoietic Progenitor CellsFlow CytometryCell DivisionCell DifferentiationCFSE LabelingCell FateHigh-throughput AnalysisCell CultureSingle-cell Analysis

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