Simultaneous Assessment of Kinship, Division Number, and Phenotype <em>via</em> Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Alessandro Donada; Tamar Tak; Giulio Prevedello; Idan Milo; Ken R. Duffy; Le&#239;la Peri&#233;

doi:10.3791/64918

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Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Journal JoVE
Immunologie et infection

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Journal JoVE Immunologie et infection

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

1,026 Views

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10:20 min

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March 24, 2023

DOI:

10.3791/64918-v

DOI:

10.3791/64918-v

•

10:20 min

March 24, 2023

•

8 Views

Alessandro Donada¹, Tamar Tak¹, Giulio Prevedello^1,2,3,4, Idan Milo¹, Ken R. Duffy², Leïla Perié¹

¹Quantitative Immuno-hematology, CNRS UMR168,Institut Curie, ²Hamilton Institute,Maynooth University, ³Institut Curie, PSL Research University, CNRS UMR 3348, Orsay, ⁴Université Paris-Sud, Université Paris-Saclay, CNRS UMR 3348, Orsay

Summary

Presented here is a flow cytometry-based technique that allows for simultaneously measuring the number of cell divisions, surface cell phenotype, and cellular kinship. Those properties can be tested statistically using a permutation-based framework.

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Hematopoietic Stem Cells Hematopoietic Progenitor Cells Flow Cytometry Cell Division Cell Differentiation CFSE Labeling Cell Fate High-throughput Analysis Cell Culture Single-cell Analysis

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Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Summary

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