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Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells

1,026 Views

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10:20 min

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March 24, 2023

DOI:

10.3791/64918-v

DOI:
10.3791/64918-v

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10:20 min
March 24, 2023

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1Quantitative Immuno-hematology, CNRS UMR168,Institut Curie, 2Hamilton Institute,Maynooth University, 3Institut Curie, PSL Research University, CNRS UMR 3348, Orsay, 4Université Paris-Sud, Université Paris-Saclay, CNRS UMR 3348, Orsay

Summary

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Presented here is a flow cytometry-based technique that allows for simultaneously measuring the number of cell divisions, surface cell phenotype, and cellular kinship. Those properties can be tested statistically using a permutation-based framework.

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Donada, A., Tak, T., Prevedello, G., Milo, I., Duffy, K. R., Perié, L. Simultaneous Assessment of Kinship, Division Number, and Phenotype via Flow Cytometry for Hematopoietic Stem and Progenitor Cells. J. Vis. Exp. (193), e64918, doi:10.3791/64918 (2023).

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