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Eine 3D-Kultivierungsmethode von Sphäroiden embryonaler und Leberzebrafischzelllinien
JoVE 신문
환경과학
Author Produced
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JoVE 신문 환경과학
A 3D Culture Method of Spheroids of Embryonic and Liver Zebrafish Cell Lines
DOI:

08:00 min

January 20, 2023

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Chapters

  • 00:05Introduction
  • 00:55Cell Culture Medium and Monolayer Cultures
  • 02:20Cell Counting with Trypan Blue Dye Exclusion Test
  • 03:07Cell Plating in ULA-Plates
  • 04:48Results: Characterization of Spheroids Developed with 3D Spheroid Method
  • 07:12Conclusion

Summary

자동 번역

Hier stellen wir ein effektives, einfaches und schnelles 3D-Kulturprotokoll für die Bildung von Sphäroiden von zwei Zebrafisch-Zelllinien (Danio rerio) vor: ZEM2S (Embryo) und ZFL (normaler Hepatozyten).

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