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Isolement non aqueux et enrichissement des trichomes glandulaires et sessiles de Cannabis sativa
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Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa
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Isolement non aqueux et enrichissement des trichomes glandulaires et sessiles de Cannabis sativa

1,670 Views

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08:18 min

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May 12, 2023

DOI:

10.3791/64798-v

DOI:
10.3791/64798-v

•

08:18 min
May 12, 2023

•

16 Views
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1Department of Vegetable Research,ARO-Volcani Center, 2Department of Vegetable Research,ARO-Newe Ya‘ar Center, 3Department of Ornamental Horticulture and Biotechnology, Institute of Plant Sciences,ARO- Volcani Center, 4Department of Fruit Science,ARO- Volcani Center

Summary

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Un protocole est présenté pour l’isolement et l’enrichissement pratiques et à haut débit des trichomes glandulaires et sessiles de Cannabis sativa. Le protocole est basé sur une extraction sèche et non tampon des trichomes en utilisant uniquement de l’azote liquide, de la glace sèche et des tamis en nylon et convient à l’extraction d’ARN et à l’analyse transcriptomique.

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Cohen, S., Itkin, M., Faigenboim, A., Davidovich-Rikanati, R., Bar, E., Hasson, D., Shalev, N., Koltai, H., Sagee, O., Lewinsohn, E., Spitzer-Rimon, B., Schaffer, A. A. Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa. J. Vis. Exp. (195), e64798, doi:10.3791/64798 (2023).

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