Journal
/
유전학
/
Niet-waterige isolatie en verrijking van glandulaire capitate gestalkte en sessiele trichomen van Cannabis sativa
JoVE 신문
유전학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 유전학
Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa
Please note that all translations are automatically generated. Click here for the English version.

Niet-waterige isolatie en verrijking van glandulaire capitate gestalkte en sessiele trichomen van Cannabis sativa

1,670 Views

•

08:18 min

•

May 12, 2023

DOI:

10.3791/64798-v

DOI:
10.3791/64798-v

•

08:18 min
May 12, 2023

•

16 Views
, , , , , , , , , , ,
1Department of Vegetable Research,ARO-Volcani Center, 2Department of Vegetable Research,ARO-Newe Ya‘ar Center, 3Department of Ornamental Horticulture and Biotechnology, Institute of Plant Sciences,ARO- Volcani Center, 4Department of Fruit Science,ARO- Volcani Center

Summary

Automatically generated

Er wordt een protocol gepresenteerd voor de handige en high-throughput isolatie en verrijking van glandulaire capitate stalked en sessiele trichomen van Cannabis sativa. Het protocol is gebaseerd op een droge, niet-bufferextractie van trichomen met alleen vloeibare stikstof, droogijs en nylonzeven en is geschikt voor RNA-extractie en transcriptomische analyse.

Explore More Videos

Non-aqueous IsolationEnrichmentGlandular Capitate Stalked TrichomesGlandular Capitate Sessile TrichomesCannabis SativaHigh ThroughputHigh ConcentrationTrichome DensityDry IceLiquid NitrogenMesh SieveExtractionPlant MaterialPlant Processing

Read Article

Cite this Article

Cohen, S., Itkin, M., Faigenboim, A., Davidovich-Rikanati, R., Bar, E., Hasson, D., Shalev, N., Koltai, H., Sagee, O., Lewinsohn, E., Spitzer-Rimon, B., Schaffer, A. A. Non-Aqueous Isolation and Enrichment of Glandular Capitate Stalked and Sessile Trichomes from Cannabis sativa. J. Vis. Exp. (195), e64798, doi:10.3791/64798 (2023).

Download .ris file

Copy

Share Video

.

Copy