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Beoordeling van intestinale transcytose van neonatale Escherichia coli bacteriëmie-isolaten
JoVE 신문
면역학 및 감염병학
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JoVE 신문 면역학 및 감염병학
Assessment of Intestinal Transcytosis of Neonatal Escherichia coli Bacteremia Isolates
DOI:

08:32 min

February 17, 2023

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Chapters

  • 00:04Introduction
  • 00:44Preparing Transcytosis Inserts with T84 Cells (Approximately 1 – 2 Weeks Before the Experiment)
  • 03:43Preparing T84 Cells 1 Day Before the Experiment Using TCM w/o Antibiotics and Escherichia coli Cultures (Started 1 Day Before the Experiment)
  • 04:19Preparing E. coli Inoculum, Epithelial Cells, and Materials (On the Morning of the Experiment)
  • 05:05Inoculation of the Cells (Start of the Experiment)
  • 05:54Quantifying the Transcytosis and End of the Experiment
  • 06:56Results: Transcytosis of Neonatal E. coli Isolates
  • 08:04Conclusion

Summary

자동 번역

Escherichia coli veroorzaakt sepsis bij pasgeborenen die de bacteriën rond het moment van geboorte binnenkrijgen. Het proces dat betrokken is bij het vermogen van E. coli om van het enterische kanaal naar de bloedbaan te reizen, is slecht begrepen. Dit in vitro model beoordeelt het vermogen van E. coli stammen om door de darmepitheelcellen te reizen.

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