JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Development of Knock-Out Muscle Cell Lines using Lentivirus-Mediated CRISPR/Cas9 Gene Editing
3,727 Views
•
10:12 min
•
June 16, 2022
DOI:
Summary
Automatically generated
The protocol describes how to generate knock-out myoblasts using CRISPR/Cas9, starting from the design of guide-RNAs to the cellular cloning and characterization of the knock-out clones.
Read Article
Cite this Article
Beaufils, M., Tourel, A., Petiot, A., Halmai, N. B., Segal, D. J., Rendu, J., Marty, I. Development of Knock-Out Muscle Cell Lines using Lentivirus-Mediated CRISPR/Cas9 Gene Editing. J. Vis. Exp. (184), e64114, doi:10.3791/64114 (2022).
Copy