Development of Knock-Out Muscle Cell Lines using Lentivirus-Mediated CRISPR/Cas9 Gene Editing

Mathilde Beaufils; Amandine Tourel; Anne Petiot; Nicole B. Halmai; Dave J. Segal; John Rendu; Isabelle Marty

doi:10.3791/64114

Journal

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Genética

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Development of Knock-Out Muscle Cell Lines using Lentivirus-Mediated CRISPR/Cas9 Gene Editing

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Genética

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Development of Knock-Out Muscle Cell Lines using Lentivirus-Mediated CRISPR/Cas9 Gene Editing

3,727 Views

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10:12 min

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June 16, 2022

DOI:

10.3791/64114-v

DOI:

10.3791/64114-v

•

10:12 min

June 16, 2022

•

2 Views

Mathilde Beaufils¹, Amandine Tourel¹, Anne Petiot¹, Nicole B. Halmai², Dave J. Segal², John Rendu¹, Isabelle Marty¹

¹University Grenoble Alpes, INSERM, U1216, CHU Grenoble Alpes, Grenoble Institut Neurosciences, ²Genome Center,University of California, Davis

Summary

The protocol describes how to generate knock-out myoblasts using CRISPR/Cas9, starting from the design of guide-RNAs to the cellular cloning and characterization of the knock-out clones.

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Development of Knock-Out Muscle Cell Lines using Lentivirus-Mediated CRISPR/Cas9 Gene Editing

Summary

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