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Development of Knock-Out Muscle Cell Lines using Lentivirus-Mediated CRISPR/Cas9 Gene Editing
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June 16, 2022
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The protocol describes how to generate knock-out myoblasts using CRISPR/Cas9, starting from the design of guide-RNAs to the cellular cloning and characterization of the knock-out clones.
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Beaufils, M., Tourel, A., Petiot, A., Halmai, N. B., Segal, D. J., Rendu, J., Marty, I. Development of Knock-Out Muscle Cell Lines using Lentivirus-Mediated CRISPR/Cas9 Gene Editing. J. Vis. Exp. (184), e64114, doi:10.3791/64114 (2022).
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