This paper describes a method for chronic in vivo observation of the resting microglia in the mouse hippocampal CA1 using precisely controlled surgery and two-photon microscopy.
Kamei, R., Urata, S., Maruoka, H., Okabe, S. In Vivo Chronic Two-Photon Imaging of Microglia in the Mouse Hippocampus. J. Vis. Exp. (185), e64104, doi:10.3791/64104 (2022).