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In Vivo Chronic Two-Photon Imaging of Microglia in the Mouse Hippocampus
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In Vivo Chronic Two-Photon Imaging of Microglia in the Mouse Hippocampus

In Vivo Chronic Two-Photon Imaging of Microglia in the Mouse Hippocampus

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07:03 min

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July 06, 2022

DOI:

10.3791/64104-v

DOI:
10.3791/64104-v

•

07:03 min
July 06, 2022

•

16 Views
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1Department of Cellular Neurobiology, Graduate School of Medicine,The University of Tokyo

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This paper describes a method for chronic in vivo observation of the resting microglia in the mouse hippocampal CA1 using precisely controlled surgery and two-photon microscopy.

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MicrogliaHippocampusIn VivoTwo-photon ImagingNeuronsCranial WindowExternal CapsuleAlveusCA1Two-photon Microscope

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Kamei, R., Urata, S., Maruoka, H., Okabe, S. In Vivo Chronic Two-Photon Imaging of Microglia in the Mouse Hippocampus. J. Vis. Exp. (185), e64104, doi:10.3791/64104 (2022).

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