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JoVE Journal Neuroscience
In Vivo Chronic Two-Photon Imaging of Microglia in the Mouse Hippocampus
 

In Vivo Chronic Two-Photon Imaging of Microglia in the Mouse Hippocampus

Article DOI: 10.3791/64104-v • 07:03 min • July 6th, 2022
July 6th, 2022 •
1, 1, 1, 1
1Department of Cellular Neurobiology, Graduate School of Medicine, The University of Tokyo

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This paper describes a method for chronic in vivo observation of the resting microglia in the mouse hippocampal CA1 using precisely controlled surgery and two-photon microscopy.

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In Vivo Chronic Two-Photon Imaging Microglia Mouse Hippocampus Direct Imaging Regulate Neurons In Vivo Imaging Observation Window Circular Groove Dermal Punch Alignment Central Bone Island Dura Picker Pia Pial Vessels Cortical Tissue Exposed Tissue Surface Cranial Window Fibers Of The External Capsule Suction Tip Lateral Ventricle Rostrolateral End Caudomedial To Rostrolateral Direction Mediolateral Direction External Capsule Removal Alveus Dorsal Hippocampal Commissure Bleeding
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